HPLC/RPC
HPLC and RPC (Reverse Phase Cartridge) purification methods yield purity of 85% to 95% depending upon the sequence, GC content and length of the oligonucletide.
Reverse phase based HPLC fails above 40 mer as longer oligos are inherently hydrophobic and bind non-specifically.
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Polyacrylamide Gel Purification (PAGE)
Purification by this method is considered the Gold Standard for oligo purification and yields 99%+ purity.
Gel purification can be used for any length of oligonucletide (as compared to HPLC and RPC cartridges which are limited to oligonucleotides below 40 mer).
Gel purification is strongly advised for all applications involving cloning of the product, such as mutagenesis and gene construction applications.
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RNA Purification (price per oligo)
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Product
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50 nmol scale
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200 nmol scale
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1 mmol scale
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2 mmol scale
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10 mmol scale
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15 mmol scale
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Gel Purification
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90.00
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90.00
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210.00
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310.00
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1900.00
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2250.00
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Catalog Number
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27-6400-77
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27-6400-77
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27-6400-55
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27-6400-88
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27-6400-44
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27-6400-99
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Reverse Phase Cartridge
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45.00
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45.00
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135.00
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270.00
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1080.00
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1380.00
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Catalog Number
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27-6400-11
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27-6400-11
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27-6400-22
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27-6400-23
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27-6400-33
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27-6400-34
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Oligo Purification