IRDye QC-1 NHS Quencher

IRDye QC-1N Quencher

Code : [IRD-QC-1]

Modification : IRDye QC-1N Quencher

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6674
Quenchers
[IRD-QC-1]
Y
Y
Y
1145.67
-
PS26-6674.pdf
750
-
-

Catalog No	Scale	Price
26-6674-05	50 nmol	$486.00
26-6674-02	200 nmol	$702.00
26-6674-01	1 umol	$1,690.00
26-6674-03	2 umol	$3,105.00
26-6674-06	5 umol	$7,605.00

Related Modifications
BBQ-650-5'	BBQ-650 Internal	IRDye 800RS-N
IRDye 700-N	BBQ-650 NHS	BBQ-650-3'
BBQ-650-dT

Click here for a list of fluorophores.

This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C3, C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

IRDye QC-1 is a non-fluorescent (dark) quencher compatible with a wide range of visible and near-infrared fluorophores (~500-800 nm). The Absorption max in 1X PBS is 737 nm and 788 nm in water.It has a wide quenching range. IRDye QC-1 quenches common fluorophores with >97% efficiency.
We recommend to consider BBQ-650 quencher as a excellent substitute. See table below.

Click here for list of quenchers.

Click here for a list of fluorophores.

Quencher Spectral Data

Quencher

Absorption Max, nm

Quenching Range, nm

Dabcyl 453 380-530

BHQ-0 495 430-520

BHQ-1 534 480-580

BHQ-2 579 550-650

BHQ-3 672 620-730

BBQ-650 650 550-750

Click here for complete list of quenchers and details

**Black Hole Quencher License Agreement
Black Hole Quencher License Agreement. "Black Hole Quencher^�, BHQ^�, CAL Fluor^� and Quasar^� are registered trademarks of Biosearch Technologies, Inc., Petaluma, California. The BHQ, CAL Fluor and Quasar dye technologies are protected by U.S. and world-wide patents either issued or in application. Compounds incorporating these dyes are made and sold under agreement with Biosearch Technologies, Inc. for end-user's non-commercial research and development use only. Their use in commercial applications is encouraged but requires a separate Commercial Use License granted by Biosearch Technologies, Inc."

Yield of Post Synthesis NHS, Maleimide & Click Ligand Conjugation*
Oligo Scale of Synthesis Yield, nmols

50 nmol 2 nmol

200 nmol 5 nmol

1 umol 16 nmol

2 umol 30 nmol

5 umol 75 nmol

10 umol 150 nmol

15 umol 225 nmol

* The yield will be lower for oligos longer than 50mer. Click here for yield table of long oligos.

* Click here for RNA Oligos scale of synthesis and yield.

NHS Ligand conjugation requires a primary amino group. Gene Link offers a wide selection of amino modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.

Maleimide Ligand conjugation requires a thiol group. Gene Link offers a wide selection of thiol modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.

Click Chemistry Ligand conjugation requires a corresponding Click modification; examples Alkyne:Azide, Azide:DBCO, BCN:Azide, BCN: TCO:Tetrazine. Gene Link offers a wide selection of click modifications for 5', 3' and internal sites. Click here for a list of click chemistry modifications.

IRDye QC-1 is a non-fluorescent (dark) quencher compatible with a wide range of visible and near-infrared fluorophores (~500-800 nm). It has a wide available quenching range with the maxima in the range of 700-800 nm. IRDye QC-1 quenches common fluorophores with >97% efficiency. It is ideal for all IR dyes. - IRDye QC-1 NHS Quencher