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Dabcyl-5'

Dabcyl-5'

Code : [Dab-5]

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Modification : Dabcyl-5'

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6704
Quenchers
[Dab-5]
Y
Y
N
462.44
11.1
PS26-6704.pdf
-
-
-

picture of Dabcyl-5'


Catalog NoScalePrice
26-6704-0550 nmol$267.00
26-6704-02200 nmol$267.00
26-6704-011 umol$347.00
26-6704-032 umol$521.00
26-6704-1010 umol$3,475.00
26-6704-1515 umol$2,780.00
Discounts are available for Dabcyl-5'!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Dabcyl-55 is classified as a dark quencher (a non-fluorescent chromophore), and is typically used to label a Fluorescence Resonance Energy Transfer (FRET) DNA oligonucleotide probe with a quencher moiety at the 5'-end. TaqMan or Molecular Beacon probes labeled with Dabcyl at the 5'-end usually would be labeled with an appropriate fluorescent dye at the 3'-end; other kinds of FRET probes might have the fluorescent dye at an internal position. 5'-end labeling such probes with Dabcyl is unusual. The primary reason for using this inverse-labeling strategy is to take advantage of Dabcyl's very high hydrophobicity (which is higher than that of a fluorescent dye) to improve subsequent purification of the probe by HPLC or RPC. For example, a molecular beacon with 5'-Dabcyl and 3'-6-FAM is easier to purify (and will subsequently be purer) than a probe with the more common, opposite set-up.


Click here for list of quenchers.

Click here for a list of fluorophores.


Quencher Spectral Data

Quencher

Absorption Max, nm

Quenching Range, nm

Dabcyl 453 380-530
BHQ-0 495 430-520
BHQ1 534 480-580
BHQ2 579 550-650
BHQ3 672 620-730
BBQ-650 650 550-750
Click here for complete list of quenchers and details
**Black Hole Quencher License Agreement
Black Hole Quencher License Agreement. "Black Hole Quencher, BHQ, CAL Fluor and Quasar are registered trademarks of Biosearch Technologies, Inc., Petaluma, California. The BHQ, CAL Fluor and Quasar dye technologies are protected by U.S. and world-wide patents either issued or in application. Compounds incorporating these dyes are made and sold under agreement with Biosearch Technologies, Inc. for end-user's non-commercial research and development use only. Their use in commercial applications is encouraged but requires a separate Commercial Use License granted by Biosearch Technologies, Inc."



- Dabcyl-5'

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