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dideoxy C-3'

ddC-3'

Code : [ddC-3]

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picture of dideoxy C-3'

Modification : ddC-3'

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6337C
End Blockers
[ddC-3]
N
Y
N
273.19
7.4
PS26-6337C.pdf
-
-
-


Catalog NoScalePrice
26-6337C-0550 nmol$165.00
26-6337C-02200 nmol$185.00
26-6337C-011 umol$215.00
26-6337C-032 umol$322.50
26-6337C-065 umol$1,417.50
26-6337C-1010 umol$1,420.00
26-6337C-1515 umol$1,765.00

Dideoxycytosine (ddC) is a dideoxyribonucleoside, and is a synthetic analog of deoxycytosine. The difference between the two is that, in ddC, both the 2'- and 3'-positions of the ribose have a hydrogen (-H) group substituted for the -OH group, whereas in dC, only the 2'-position is so substituted.

ddC is generally used in two applications. First, to block the 3'-end of oligonucleotides to prevent their extension by a polymerase in a PCR reaction or PCR-based assay. For example, 3'ddC-blocked oligos have been used in microarray-based DNA re-sequencing (1). ddC is also used to block the 3'-end of 5'-adenylated oligos (5'-App) that are used as adapters in miRNA library generation. Blocking the 3'-end in this manner prevents the oligo from either circularization (by self-ligation) or concatemerization to other 5'-App oligos (2).

References
1. Sram, J., Sommer, S.S., Liu, Q. Microarray-based DNA re-sequencing using 3' blocked primers. Anal. Biochem. (2008), 3744: 41-47.
2. Lau, N.C., Lim, L.P., Weinstein, E.G., Bartel, D.P. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans.Science (2001), 294: 858-862.
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