In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Quality Control | Purification | Duplex Stability Mods | Modifications | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorer™ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBase™ siRNA Modifications | shRNA Explorer™
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemer™ | GeneProber™ | Prober™ Gene Detection Kits | GScan™ Gene Detection Kits | Genemer™ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorer™ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBase™ siRNA Modifications | shRNA Explorer™
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemer™ | GeneProber™ | Prober™ Gene Detection Kits | GScan™ Gene Detection Kits | Genemer™ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
Genetic Tools and Reagents
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Purification of DNA from
agarose gels and concentration
of DNA by ethanol precipitation
are routine protocols used in all
molecular biology laboratories.
The Omni-CleanTM system provides
optimized reagents for
rapid extraction of DNA from
agarose gels, and for routine
concentration of DNA. The DNA
is concentrated, purified and
completely salt-free.
Application - Purification of DNA fragments excised from agarose gels
The Omni-CleanTM DNA Purification
System takes advantage of the principle
that DNA binds to powdered
flint glass in the presence of
chaotropic salts. This technique provides
a rapid and efficient method for
the purification of high quality DNA
from solutions or agarose gel slices,
and is suitable for cloning, sequencing,
isotope labeling, and a host of
other procedures.
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 |
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Lanes 2 and 3 are fragments excised
from agarose gel and purified using the
Omni-CleanTM column based purification
system.
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Lane 1 is plasmid extracted using
Omni-PureTM plasmid purification system.
Lane 2 is the lower fragment gel
purified using the Omni-CleanTM gel
glass bead based purification system.
|
Application : Concentration and Cleanup of DNA in solution
The Omni-CleanTM DNA
Concentration System yields higher
quality DNA than with conventional
ethanol precipitation. The samples
are completely desalted, purified
and concentrated. It is ideal for
sequencing and genotyping samples
with stringent purity requirements.
The electropherogram shows samples
purified using the Omni-CleanTM
system.