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5-Hydroxymethyl-dU

picture of 5-Hydroxymethyl-dU

Modification : 5-hm dU

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6898
Epigenetics
5-hm dU
Y
Y
Y
320.19
8.4
PS26-6898.pdf
-
-
-


Catalog NoScalePrice
26-6898-0550 nmol$247.50
26-6898-02200 nmol$247.50
26-6898-011 umol$321.75
26-6898-032 umol$482.62
26-6898-1010 umol$2,574.00
26-6898-1515 umol$3,217.50
Discounts are available for 5-hm dU!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

5-Hydroxymethyl deoxyuridine (5-hm-dU) is a minor DNA base; its presence in DNA strands occurs by either oxidative attack via peroxide radicals, or ionizing radiation, on the 5-methyl group of thymine (1,2). Available evidence does not support 5-hm-dU being mutagenic; however, base excision repair enzymes specific to it (i.e., hydroxymethyluracil-DNA glycosylases) are known to exist in protists and animals (3), suggesting that this lesion nevertheless may have mutagenic potential. Incorporation of 5-hm-dU into synthetic oligos for use in studie into the molecular genetics and enzymology of DNA base excision repair pathways.

However, because 5-hm-dU also appears as a deamination intermediate during the oxidative de-methylation of 5-methyl-dC to dC, 5-hm-dU can be used in studies into the role of 5-methyl-dC de-methylation in epigenetic regulation.

References
1. Cadet. J., Berger, M., Douki, T., Ravanat, J-L. Oxidative damage to DNA : formation, measurement, and biological significance.Rev. Physiol. Biochem. Pharmacol. (1997), 131: 1-87.
2. Teebor, G.W., Frenkel, K., Goldstein, M.S. Ionizing radiation and tritium transmutation both cause formation of 5-hydroxymethyl-2-deoxyuridine in cellular DNA.Proc. Natl. Acad. Sci. USA. (1984), 81: 318-321.
3. Levy, D.D., Teebor, G.W. Site directed substitution of 5-hydroxymethyluracil for thymine in replicating phiX-174am3 DNA via synthesis of 5-hydroxymethyl-2-deoxyuridine-5-triphosphate.Nucleic Acids Res. (1991), 19: 3337-3343.
- 5-Hydroxymethyl-dU

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