PCRProber™ alkaline phosphatase
labeled probe is for
amplification and non-radioactive
detection of a trinucleotide
repeat region amplified PCR
product. The PCRProber™ Kit
comprises of a primer pair for
PCR amplification followed by
gel blot and chemiluminescent
detection using the alkaline
phosphatase oligonucleotide
probe. Quantity supplied is
sufficient for 100 amplifications.
Gene Link’s PCRProber™ Kit is
based on PCR amplification followed
by Southern blot chemiluminescent
detection using an alkaline phosphatase
labeled oligonucleotide
probe. This kit is a safe and sensitive
alternate to radioactive-based detection
methods. The amplified products
are resolved on a sequencing polyacrylamide
gel, and then blotted and
processed for chemiluminescent
detection.
The PCRProber™ Kit is simple and
robust for routine triple repeat detection
of greater than 100 repeats of all
triple repeat disorders listed, except
Fragile X. The CGG repeat in Fragile
X can be detected up to ~50 repeats.
It is strongly recommended that the
genotyping be followed up by using
GeneProber™ Southern blot detection
methods when two alleles are
not clearly discernable.
