GScan™ Kits contain optimized
PCR amplification reagents and
a wide selection of fluorescentlabeled
primers for genotyping
after PCR using fluorescent
genetic analyzer instrument(s).
Kit includes sufficient reagents
for 100 detections.
Genotyping using this kit
requires use of the appropriate
fluorescent genetic analyzer
instrument(s) and software
capable of detection of fluorescently
labeled fragments of
varying lengths. These kits
have been optimized for an
ABI310 genetic analyzer.
Gene Link’s GScan™ gene detection
kits are safe, convenient and sensitive,
and afford automated compilation
of data. The kits contain optimized
PCR amplification reagents and a wide
array of fluorescent-labeled primers for
genotyping after PCR using fluorescent
genetic analyzer instrument(s).
Included in these kits are ready-to-run
control samples of various repeats of
the triple repeat disorder. These control
samples are for calibration with
the molecular weight markers for
accurate size determination of the
amplified fragments.
The GScan™ Kits are simple and
robust for routine triple-repeat detection
of greater than 100 repeats of all
triple repeat disorders listed, except
Fragile X. The CGG repeat in Fragile X
can be detected up to ~50 repeats.
It is strongly recommended that the
genotyping be followed up by using
Southern blot detection methods when
two alleles are not clearly discernable.