Genemer™ Control DNA are
cloned fragments of a particular
gene for use with gene or
mutation specific Genemer™
and GScan™ products. These
control DNAs are ideal genotyping
templates for optimizing and
performing control amplification
with unknown DNA. One tube
is supplied containing 500 ng of
lyophilized DNA segment of the
specified fragment spanning the
mutation region. The quantity
supplied is sufficient for 1000
regular 50 µl PCR reactions.
The control DNAs were developed to
complement Gene Link’s gene detection
system product line. The control
DNAs for a single base mutation are
helpful to optimize the protocol and in
setting up positive control standards.
The control DNA series for various
triple repeat disorders were generated
by amplification. These serve as
controls for the amplification of various
triple repeats and to run as positive
controls. The size of the triple
repeats has been determined by
sequencing and gel electrophoresis.
The stability of size repeats upon
cloning and amplification has not been
determined. Thus, the size should be
considered approximate and there is
no claim for each fragment to contain
the exact number of triple repeats.
These control DNAs are sold with the
expressed condition that these not be
used for exact triple repeat size determination
of DNA of unknown genotype.
The control DNA should be
used for determining the performance
of specific Genemer™, GScan™ and
PCRProber™ Gene Link products.
