 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
||||
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
|
|||||||||||||||
| Gene Detection Systems |
| ||||||||
|
GeneProber™
A specific gene fragment probe
for Southern blot based
hybridization of genomic DNA.
The GeneProber™ is available
unlabeled for radioactive based
methods and labeled with
digoxigenin for chemiluminescent
detection. One tube is
supplied containing 500 ng of
the lyophilized unlabeled
GeneProber™ probe.
The quantity supplied is sufficient
for at least 5 random
prime labeling reactions using
100 ng for each reaction. Gene
Link recommends using 25 ng
of probe for each labeling reaction.
The digoxigenin labeled
probe supplied is sufficient for
five 20 x 20 blots.
|
|||||||||||||||||||||||||||||||||||||
|
The GeneProber™ product line is
based on the chemiluminescent
Southern blot detection method.
These probes are specially developed
to detect the triple repeat amplifications.
Fragile X, Huntington disease,
Myotonic dystrophy, Friedreich’s
ataxia and Kennedy disease
GeneProber™ unlabeled and digoxigenin
labeled are available. Gene Link’s non-radioactive detection systems for genotyping of triple repeat disorders are rapid, reliable and as sensitive as the 32P labeled southern blots. No more decayed probes and radioactive exposure. A detailed manual accompanies the product giving a step by step protocol, results and interpretation guidelines. |
|||||||||||||||||||||||||||||||||||||
 |
Fragile X CGG repeats genotyping results using Gene Link’s GeneProber™ products. Fragile X GeneProber™ GLFXDig1 Digoxigenin-labeled Probe (Catalog No. 40-2004-41) and GeneProber™ GLFX1 Unlabeled Probe (Catalog No. 40-2004-40) were used to probe human blood genomic DNA digested with Eco RI and Eag I. Left blot was hybridized with digoxigenin labeled probe and processed for chemiluminescent detection. The blot on the right was hybridized with 32P labeled GLFX1 and processed for radioactive Southern blot detection. Both blots were exposed overnight to X-ray films. Normal fragment sizes for females are between 2.8 kb and 5.2 kb, and a single fragment of 2.8 kb in males. The blot shows samples with CGG repeats in the affected range. | ||||||||||||||||||||||||||||||||||||
|
|
||||||||||||||||||||||||||||||||||||