CF-405M Dye NHS

CF-405M Dye N

Code : [CF-405M-N]

Modification : CF-405M Dye N

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6813
Fluorescent Dyes
[CF-405M-N]
Y
Y
Y
503
41000
PS26-6813.pdf
416
452
41000

Catalog No	Scale	Price
26-6813-05	50 nmol	$550.00
26-6813-02	200 nmol	$620.00
26-6813-01	1 umol	$813.00
26-6813-03	2 umol	$1,410.00
26-6813-10	10 umol	$5,040.00
26-6813-15	15 umol	$6,040.00

Related Modifications
AZDye-488-N	CF-405S Dye N	AZDye-546-N
AZDye-532-N	CF-450 Dye N	AZDye-430-N
CF-430 Dye N

Click here for a list of fluorophores.

See detailed technical specifications of the CF Dyes. Biotium CF Dyes Technical Specifications.

Biotium CF Dyes Reference Guide .

This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C3, C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

Yield of Post Synthesis NHS, Maleimide & Click Ligand Conjugation*
Oligo Scale of Synthesis	Yield, nmols
50 nmol	2 nmol
200 nmol	5 nmol
1 umol	16 nmol
2 umol	30 nmol
5 umol	75 nmol
10 umol	150 nmol
15 umol	225 nmol
* The yield will be lower for oligos longer than 50mer. Click here for yield table of long oligos.
* Click here for RNA Oligos scale of synthesis and yield.
NHS Ligand conjugation requires a primary amino group. Gene Link offers a wide selection of amino modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Maleimide Ligand conjugation requires a thiol group. Gene Link offers a wide selection of thiol modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Click Chemistry Ligand conjugation requires a corresponding Click modification; examples Alkyne:Azide, Azide:DBCO, BCN:Azide, BCN: TCO:Tetrazine. Gene Link offers a wide selection of click modifications for 5', 3' and internal sites. Click here for a list of click chemistry modifications.

CF Dyes (CF is a registered trademark of Biotium, Inc.) are a series of highly water-soluble fluorescent dyes spanning the visible and near-infrared spectrum for labeling biomolecules. These are available over a wide range of visible and near IR range. These offer superior brightness, photostability, and signal-to-noise detection.

CF Dyes are available as amine-reactive forms that is used to conjugate them to a primary amine modifications in an oligo.
CF is a registered trademark of Biotium, Inc.

- CF-405M Dye NHS