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dideoxy A-3'


Code : [ddA-3]

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picture of dideoxy A-3'

Modification : ddA-3'

Catalog Reference Number
Modification Code
5 Prime
3 Prime
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

End Blockers

Catalog NoScalePrice
26-6337A-0550 nmol$485.00
26-6337A-02200 nmol$485.00
26-6337A-011 umol$515.00
26-6337A-032 umol$636.00
26-6337A-1010 umol$2,715.00
26-6337A-1515 umol$3,356.00

Dideoxycytosine (ddA) is a dideoxyribonucleoside, and is a synthetic analog of deoxyadenosine. The difference between the two is that, in ddA, both the 2'- and 3'-positions of the ribose have a hydrogen (-H) group substituted for the -OH group, whereas in dA, only the 2'-position is so substituted.

ddA is generally used in two applications. First, to block the 3'-end of oligonucleotides to prevent their extension by a polymerase in a PCR reaction or PCR-based assay. For example, 3'ddA-blocked oligos can be used in microarray-based DNA re-sequencing (1). ddC is also used to block the 3'-end of 5'-adenylated oligos (5'-App) that are used as adapters in miRNA library generation. Blocking the 3'-end in this manner prevents the oligo from either circularization (by self-ligation) or concatemerization to other 5'-App oligos (2).

1. Sram, J., Sommer, S.S., Liu, Q. Microarray-based DNA re-sequencing using 3' blocked primers. Anal. Biochem. (2008), 3744: 41-47.
2. Lau, N.C., Lim, L.P., Weinstein, E.G., Bartel, D.P. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans.Science (2001), 294: 858-862.
- dideoxy A-3'

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