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7-deaza-dA

7-deaza-dA

Code : [7-deaza-dA]

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picture of 7-deaza-dA

Modification : 7-deaza-dA

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6916
Structural Studies
[7-deaza-dA]
Y
Y
Y
312.22
15.4
PS26-6916.pdf
260
-
15.4


Catalog NoScalePrice
26-6916-0550 nmol$383.00
26-6916-02200 nmol$383.00
26-6916-011 umol$498.00
26-6916-032 umol$748.00
26-6916-065 umol$2,241.00
26-6916-1010 umol$3,987.00
26-6916-1515 umol$4,984.00
Discounts are available for 7-deaza-dA!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

7-deaza dA has a nitrogen at position 7 replaced by a carbon. [7-deaza-8-aza-deoxyadenosine (7-deaza-8-aza-dA) is a deoxyribonucleoside in which the 7-nitrogen and 8-carbon are flipped. The resulting modified dA is unable to form a hydrogen bond at position 7, but can at position 8, of the base. The result is that the 7-deaza-8-aza-dA : dT base pair has essentially the same duplex stability as that of the unmodified A : T base pair, and is more stable than the 7-deaza-dA : dT base pair (1). Similar to 7-deaza-dA, 7-deaza-8-aza-dA can be used for modulate the amount of structural DNA bending existing within long polyA regions of single- and double-stranded oligonucleotides (2).

Furthermore, 7-deaza-8-aza-dA is specifically recommended over 7-deaza-dA whenever multiple insertions of a 7-deaza-dA-type modification into an oligo must be done. This is because 7-deaza-8-aza-dA is stable to the iodine-based oxidizer solution used in phosphoramidite-based DNA synthesis, while 7-deaza-dA is sensitive to it.

References
1. Seela, F.; Zulauf, M. Synthesis of oligonucleotides containing pyrazola[3,4-d]pyrimidines: The influence of 7-substituted 8-aza-7-deazaadenines on the duplex structure and stability. J. Chem. Soc., Perkin Trans. (1999), 1: 479-488.
2. Seela, F.; Grein, T. 7-Deaza-2'-deoxyadenosine and 3-deaza-2'-deoxyadenosine replacing dA within d(A6)-tracts: differential bending at 3'- and 5'-junctions of d(A6)-d(T6) and B-DNA.Nucleic Acids Res. (1992), 20: 2297-2306.
- 7-deaza-dA

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