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Tyramide Oligo

Tyramide C10 Oligo

Code : [Tyr-C10]

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Modification : Tyramide C10 Oligo

Catalog Reference Number
Modification Code
5 Prime
3 Prime
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
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Affinity Ligands

Catalog NoScalePrice
26-6681-0550 nmol$330.00
26-6681-02200 nmol$330.00
26-6681-011 umol$448.00
26-6681-032 umol$578.00
26-6681-1010 umol$1,963.00
26-6681-1515 umol$2,655.00
Discounts are available for Tyramide C10 Oligo!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Tyramide-conjugated DNA barcodes & Tyramide Signal Amplification

See a report by Paul Simonson, Itzel Valencia, Sanjay S. Patel. Indexable signal amplification for multiparametric imaging. in bioRxiv preprint.

Multiparametric imaging allows researchers to measure the expression of many biomarkers simultaneously, allowing detailed characterization of cell microenvironments. One such technique, CODEX, allows fluorescence imaging of >30 proteins in a single tissue section. In the commercial CODEX system, primary antibodies are conjugated to DNA barcodes. This modification can result in antibody dysfunction, and development of a custom antibody panel can be very costly and time consuming as trial and error of modified antibodies proceeds. To address these challenges, we developed novel tyramide-conjugated DNA barcodes that can be used with primary antibodies via peroxidase-conjugated secondary antibodies. This approach results in signal amplification and imaging without the need to conjugate primary antibodies. When combined with commercially available barcode-conjugated primary antibodies, working antibody panels can be quickly developed. This report presents methods to perform antibody staining using a commercially available automated tissue stainer and in situ hybridization imaging on a CODEX platform.

In this report, they authors present a generalizable method for CODEX imaging with unmodified primary antibodies and signal amplification that uses novel tyramide-conjugated DNA barcodes (tyramide-barcodes). This allows the benefits of tyramide signal amplification, but image the targets in an indexed way using a commercial CODEX imaging system. This approach is flexible, and demonstrate that it can be used with in situ hybridization probes. In order to make this system workable, they developed a staining approach that allowed to perform both tyramide-barcode staining and standard CODEX staining using a commercially available automated tissue stainer, made possible using a custom coverslip holder. This drastically reduces the amount of manual labor required for CODEX staining and has the potential to improve staining reproducibility.

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