LC Cyan 500 NHS

LC Cyan 500-N

Code : [LC-C500-N]

Modification : LC Cyan 500-N

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6688
Fluorescent Dyes
[LC-C500-N]
Y
Y
Y
580
-
PS26-6688.pdf
450
495
450

Catalog No	Scale	Price
26-6688-05	50 nmol	$737.00
26-6688-02	200 nmol	$737.00
26-6688-01	1 umol	$1,683.00
26-6688-06	5 umol	$4,573.50

Related Modifications
CAL Fluor Red 590	LC Red 610 N	CAL Fluor Red 610
CAL Fluor Red 635	LC Red 640 N	LC Red 705 N

Click here for a list of fluorophores.

This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C3, C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

Yield of Post Synthesis NHS, Maleimide & Click Ligand Conjugation*
Oligo Scale of Synthesis	Yield, nmols
50 nmol	2 nmol
200 nmol	5 nmol
1 umol	16 nmol
2 umol	30 nmol
5 umol	75 nmol
10 umol	150 nmol
15 umol	225 nmol
* The yield will be lower for oligos longer than 50mer. Click here for yield table of long oligos.
* Click here for RNA Oligos scale of synthesis and yield.
NHS Ligand conjugation requires a primary amino group. Gene Link offers a wide selection of amino modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Maleimide Ligand conjugation requires a thiol group. Gene Link offers a wide selection of thiol modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Click Chemistry Ligand conjugation requires a corresponding Click modification; examples Alkyne:Azide, Azide:DBCO, BCN:Azide, BCN: TCO:Tetrazine. Gene Link offers a wide selection of click modifications for 5', 3' and internal sites. Click here for a list of click chemistry modifications.

Cyan 500 NHS ester is an amine-reactive fluorescent label. It is designed and recommended by Roche for application in LightCycler� instruments. The dye is used to label amino-modified oligonucleotides which can be detected on excitation at 430 nm in Roche LightCycler� instruments.
Fluorescence properties of LC Cyan 500 has an Excitation max of 436 nm and Emission max at 481 nm. A close substitution will be AF488 with Excitation max of 494 nm and Emission max at 517 nm or Fam Excitation max of 495 nm and Emission max at 520 nm. - LC Cyan 500 NHS