Atto Rho101 NHS

Atto Rho101-N

Code : [AttoRho101-N]

Modification : Atto Rho101-N

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6970
Fluorescent Dyes
[AttoRho101-N]
Y
Y
Y
892
-
PS26-6970.pdf
586
610
-

Catalog No	Scale	Price
26-6970-05	50 nmol	$702.00
26-6970-02	200 nmol	$702.00
26-6970-01	1 umol	$913.00
26-6970-03	2 umol	$2,754.00
26-6970-06	5 umol	$4,108.50
26-6970-10	10 umol	$7,301.00
26-6970-15	15 umol	$9,126.00

Click here for a list of fluorophores.

This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C3, C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

Yield of Post Synthesis NHS, Maleimide & Click Ligand Conjugation*
Oligo Scale of Synthesis	Yield, nmols
50 nmol	2 nmol
200 nmol	5 nmol
1 umol	16 nmol
2 umol	30 nmol
5 umol	75 nmol
10 umol	150 nmol
15 umol	225 nmol
* The yield will be lower for oligos longer than 50mer. Click here for yield table of long oligos.
* Click here for RNA Oligos scale of synthesis and yield.
NHS Ligand conjugation requires a primary amino group. Gene Link offers a wide selection of amino modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Maleimide Ligand conjugation requires a thiol group. Gene Link offers a wide selection of thiol modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Click Chemistry Ligand conjugation requires a corresponding Click modification; examples Alkyne:Azide, Azide:DBCO, BCN:Azide, BCN: TCO:Tetrazine. Gene Link offers a wide selection of click modifications for 5', 3' and internal sites. Click here for a list of click chemistry modifications.

ATTO Rho101 is a derivative of the well-known dye Rhodamine 101. Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO Rho101 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. - Atto Rho101 NHS