Modification : Puromycin
Catalog Reference Number
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Puromycin can be attached to the 3' end of RNA and DNA oligos. Puromycin is an antibiotic that mimics transfer RNA. Puromycin binds in the ribosome's A site and forms a peptide bond with the growing peptide chain to block peptide elongation. By linking puromycin to synthetic RNA; a peptide-RNA fusion product can be formed.
An application example is the use of 3'Puromycin to synthesize d(A27CC)-puromycin to which various mRNA sequences were then ligated. The mRNA sequence information was then translated in a reticulocyte lysate system. As the ribosome reached the poly-dA sequence, translation was stalled. Puromycin entered the ribosome A site and a peptide bond formed between the C-terminal of the synthesized peptide and the RNA encoding the peptide structure. The poly-dA sequence serves two purposes, first it stalls the ribosome thereby allowing puromycin to enter the A site and second it acts as a future capture site for oligo-dT-biotin.
(1) S. Borman, C&EN, Feb. 12, 1996, 29-54.
(2) R.W. Roberts and J.W. Szostak, Proc. Natl. Acad. Sci. USA, 1997, 94, 12297-302