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Modification : PC Linker (photocleavable)
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
| Catalog No | Scale | Price |
| 26-6888-05 | 50 nmol | $313.00 |
| 26-6888-02 | 200 nmol | $313.00 |
| 26-6888-01 | 1 umol | $356.00 |
| 26-6888-03 | 2 umol | $443.00 |
| 26-6888-10 | 10 umol | $2,851.00 |
| 26-6888-15 | 15 umol | $3,564.00 |
| Discounts are available for PC Linker (photocleavable)! |
| Modification* Discount Price Structure |
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1 site/order
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List price
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2 sites/order
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10% discount
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3 sites/order
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20% discount
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4 sites/order
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30% discount
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5-9 sites/order
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50% discount
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10+ sites/order
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60% discount
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*Exceptions apply
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PC Linker (photocleavable) is a non-nucleosidic molety that can be used to link two nucleotide sequences through a short, UV photo-cleavable C3 spacer arm, this can be added at any position of the sequence. Photo-cleavage of PC Linker by UV light yields one 5'-phosphorylated oligo and one 3'-phosphorylated oligo.
The utility of PC Linker for photo-triggered hybridization applications was first demonstrated by Ordoukhanian and Taylor in 1995 (1). They incorporated PC Linker into the sugar-phosphate backbone of a DNA hairpin. Upon irradiation by UV light, photo-cleavage released a 5-phosphorylated 18-mer oligonucleotide having 9X greater hybridization affinity for a complementary DNA strand.
The use of PC Linker has also been explored in designing multi-functional single-stranded nucleotide conjugates for use in in vitro selection of novel DNA or RNA-based catalysts for bio-molecular or organic reactions (for example Diels-Alder) (2,3). PC Linker-modified oligonucleotides are the centerpiece of Bruker Daltonik's genoSNIP, a MALDI-TOF MS based assay system for SNP detection (4).
Cleavage Protocol
Cleavage occurs by irradiation with near-UV light (300-350 nm, complete cleavage occurs within 5 minutes. Try using a Black Ray XX-15 UV lamp (Ultraviolet Products Inc., San Gabriel, CA) at a distance of 15 cm (emission peak 365 nm, 300 nm cut-off, 1.1 mW intensity at~31 cm).
References
1. Olejnik, J., Krzymanska-Olejnik, E., Rothschild, K.J. Photocleavable aminotag phosphoramidites for 5'-termini DNA/RNA labeling.
Nucleic Acids Res. (1998),
26: 3572-3576.
2. Olejnik, J., Ludemann, H-C., Olejnik, E.K, Berkenkamp, S., Hillenkamp, F., Rothschild, K.J. Photocleavable peptide-DNA conjugates: synthesis and applications to DNA analysis using MALDI-MS.
Nucleic Acids Res. (1999),
27: 4626-4631.
3. Tang, X., Su, M., Yu, LiLi, Lv, C., Wang, J., Li, Z. Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides.
Nucleic Acids Res. (2002),
38: 3848-3855.
- PC Linker (photocleavable)
Oligo Purification