8-amino-dA

Code : [8-am-dA]

Modification : 8-amino-dA

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6535
Duplex Stability
[8-am-dA]
Y
Y
Y
328.22
15.4
PS26-6535.pdf
1
1
15.4

Catalog No	Scale	Price
26-6535-05	50 nmol	$450.00
26-6535-02	200 nmol	$450.00
26-6535-01	1 umol	$565.00
26-6535-03	2 umol	$748.00
26-6535-10	10 umol	$3,987.00
26-6535-15	15 umol	$4,984.00

Discounts are available for 8-amino-dA!
Modification* Discount Price Structure
1 site/order	List price
2 sites/order	10% discount
3 sites/order	20% discount
4 sites/order	30% discount
5-9 sites/order	50% discount
10+ sites/order	60% discount
*Exceptions apply

8-Amino-deoxyAdenosine (8-Amino-dA) is an 8-amino-purine that is most commonly used to study the structural and functional properties of triple helices. The 8-amino group is able to form an additional Hoogsteen purine-pyrimidine hydrogen bond, which serves to increase triple helix stability (1). Moreover, it has been shown that triple-helix-forming oligos (TFO) containing 8-amino-dA form stable helices at neutral pH, instead of under acidic conditions (2). This property may be relevant for design of TFOs slated for in vivo work, which generally occurs under physiological pH (7.3-7.4) conditions.

References
1. Cubero, E., Avino, A., de la Torre, B.G., Frieden, M., Eritja, R., Luque, F.J., Gonzalez, C., Orozco, M. Hoogsteen-based parallel-stranded duplexes of DNA. The effect of 8-amino derivatives. J. Am. Chem. Soc. (2002), 124: 3133-3142.
2. Zhu, Q., Delaney, M.O., Greenberg, M.M. Observation and elimination of N-acetylation of oligonucleotides prepared using fast-protecting phosphoramidites and ultra-mild deprotection. Bioorg. Med. Chem. Lett. (2001), 11: 11105-1107.
- 8-amino-dA