TET Azide

Modification : TET-Azide

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6724
Click Chemistry
[TET-N3]
Y
Y
Y
596.2
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PS26-6724.pdf
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-
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Catalog No	Scale	Price
26-6724-05	50 nmol	$450.00
26-6724-02	200 nmol	$450.00
26-6724-01	1 umol	$877.50
26-6724-03	2 umol	$877.50
26-6724-10	10 umol	$4,680.00
26-6724-15	15 umol	$5,850.00

TET (Tetrachloro fluorescein)-Azide is a fluorescent dye containing a terminal azide group. TET has an absorbance maximum of 522 nm and an emission maximum of 538 nm. The presence of the azide allows the user to use “Click Chemistry” (a [3+2] cycloaddition reaction between alkynes and azides, using copper (I) iodide as a catalyst) to conjugate the TET-Azide to a terminal alkyne-modified oligo with extremely high regioselectivity and efficiency (1,2). Preparation of the alkyne-modified oligo can be achieved using the 5’-Hexynyl modifier (see its respective tech sheet for details).

References
1. Huisgen, R. Angew. Chem. Int. Ed. (1963), 2: 565-568.
2. Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B. A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective Ligation of Azides and Terminal Alkynes. Angew. Chem. Int. Ed. (2002), 41: 2596-2599.
- TET Azide