The Omni-Pure™ Genomic
DNA purification system is
designed for a convenient
volume of 300 µl whole blood
(lower volumes can also be
used) to yield an average of
~10 µg ultra pure DNA. This
quantity is sufficient for
restriction-based Southern
blot analysis and hundreds
of PCR-based analyses.
The Omni-Pure™ Genomic DNA
purification system is designed for
convenience and consistency. It is a
universal genomic DNA purification
system. Ultra pure genomic DNA can
be purified from small amounts of
almost all known sample types and
sources. Samples from human
blood, bodily fluids, animal and plant
tissue and microbial and viral sources
have been purified using the Omni-
Pure™ Genomic DNA purification
system. One purification is usually
sufficient to yield enough DNA for all
molecular biology applications.
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| Purified genomic DNA (~200 ng) was
electrophoresed on a 0.8% agarose gel
and stained with ethidium bromide.
Observe high quality genomic DNA that
ranges from ~30 to 50 kb in size. Lane
M contains molecular weight markers
from 10 kb to 50 bp in length. Lanes 1-4
are genomic DNA samples obtained
from blood samples of 4 different individuals
using the Omni-Pure™ system. |
Application : Blood DNA Purification
Each purification sample volume is
specially geared towards the desired
downstream application. A sample
volume of 300 µl is recommended for
human blood samples yielding on
average from 5–15 µg of high molecular
weight and high quality genomic
DNA for two restriction digestions for
Southern blot analysis. The yield is
sufficient for hundreds of PCR amplification
reactions. An accompanying
product manual contains a detailed
protocol for the extraction of genomic
DNA from tissues and bodily fluids.
 |
| Human blood genomic DNA from different
individuals purified using the Omni-Pure™
Genomic DNA Purification System.
Approximately 5 µg were digested with
different restriction enzymes and the samples
were electrophoresed on a 0.7% agarose gel.
Note the high molecular weight DNA and the
consistency between different samples. The
gel was processed for Southern blot analysis
and chemiluminescent detection. |
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Omni-Pure™ Genomic DNA Purification Systems
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Product
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Size
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Catalog No.
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Price($)
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Omni-Pure™ Blood DNA Purification System
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100
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40-4010-01
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80.00
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Omni-Pure™ Blood DNA Purification System
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500
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40-4010-05
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320.00
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Omni-Pure™ Blood DNA Purification System
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1000
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40-4010-10
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495.00
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Click here to order!
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Application : Tissue DNA Purification
An accompanying product manual
contains a detailed protocol for the
purification of genomic DNA from
animal tissue. The protocol has been
tested and yields high quality DNA.
The gel picture shows genomic DNA
extracted from mouse and rat skeletal
muscle and liver. This system is
geared towards minute tissue samples.
From 2 mg of tissue an average
yield of 2-10 µg is expected. The
DNA is suitable for all molecular
biology applications
