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Genetic Tools and Reagents

Purification of DNA from agarose gels and concentration of DNA by ethanol precipitation are routine protocols used in all molecular biology laboratories. The Omni-CleanTM system provides optimized reagents for rapid extraction of DNA from agarose gels, and for routine concentration of DNA. The DNA is concentrated, purified and completely salt-free.

Application - Purification of DNA fragments excised from agarose gels

The Omni-CleanTM DNA Purification System takes advantage of the principle that DNA binds to powdered flint glass in the presence of chaotropic salts. This technique provides a rapid and efficient method for the purification of high quality DNA from solutions or agarose gel slices, and is suitable for cloning, sequencing, isotope labeling, and a host of other procedures.

Lanes 2 and 3 are fragments excised from agarose gel and purified using the Omni-CleanTM column based purification system.

Lane 1 is plasmid extracted using Omni-PureTM plasmid purification system. Lane 2 is the lower fragment gel purified using the Omni-CleanTM gel glass bead based purification system.

Omni-CleanTM Gel DNA Purification Systems
Product Size Catalog No.
Omni-CleanTM Gel DNA Beads Purification System 100 40-4110-10
Omni-CleanTM Gel DNA Beads Purification System 500 40-4110-50
Omni-CleanTM Gel DNA Spin Column Purification System 100 40-4120-10
Omni-CleanTM Gel DNA Spin Column Purification System 500 40-4120-50

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Application : Concentration and Cleanup of DNA in solution

The Omni-CleanTM DNA Concentration System yields higher quality DNA than with conventional ethanol precipitation. The samples are completely desalted, purified and concentrated. It is ideal for sequencing and genotyping samples with stringent purity requirements. The electropherogram shows samples purified using the Omni-CleanTM system.

Omni-CleanTM Gel DNA Concentration Systems
Product Size Catalog No.
Omni-CleanTM Gel DNA Beads Concentration System 100 40-4130-10
Omni-CleanTM Gel DNA Beads Concentration System 500 40-4130-50
Omni-CleanTM Gel DNA Spin Column Concentration System 100 40-4140-10
Omni-CleanTM Gel DNA Spin Column Concentration System 500 40-4140-50

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