Omni-cDNA™ First Strand cDNA
First strand cDNA is useful for
amplifying a particular cDNA
using PCR. Gene Link provides
a convenient source of high
quality first strand cDNA
prepared from freshly obtained
tissue and appropriately frozen
during transportation.
APPLICATION:
RT-PCR , cDNA amplification,
cloning, tissue-specific gene
expression, etc.
Tissue-specific individual first strand
cDNA is available from Gene Link. These
are of high quality and lot tested for
amplification of ß -actin and/or GAPDH
cDNA fragments up to 1.1 kb in length.
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GAPDH gene and mRNA sequences showing the position of
different primers. The primers (RT-PCRmer™) were constructed
to assess the quality of Gene Link Omni-cDNA™ first strand
cDNA. Full length and near full length cDNA will amplify the 1.1
kb cDNA fragment signifying high quality. All Omni-cDNA™ lots
undergo this qualification.
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Amplification was performed
using 50 ng of Omni-cDNA™
Human Pooled First Strand
cDNA (Catalog No. 10-0100-
05) as the template. A 10 µl
aliquot of the amplified fragment
was loaded on a 1%
agarose gel. The amplified
fragment sizes obtained with
the RT-PCRmer™ primer pair
used are as follows:
lane 2, F1/R1 = 109 bp;
lane 3, F2/R2 = 208 bp;
lane 4, F1/R2 = 505 bp and
lane 5, F1/R3 = 1009 bp.
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APPLICATION:
RT-PCR, cDNA cloning, etc.
The first strand cDNA has been prepared
from pooled and or amplified mRNA
obtained from different tissues. These
are not from cultured cell lines. The
assortment of tissues vary, including
lung, heart, brain, spleen, skeletal
muscle, smooth muscle, ovaries, pancreas,
liver and kidney. The amount
supplied is sufficient for at least 50
amplifications. Each lot is tested for
amplification of ß -actin cDNA.
Omni-cDNA™ pooled first strand size
distribution is from ~5 kb to 200 bp.
These can also be used for cloning
mRNA of interest by RT-PCR. A 1.3 kb
and a ~500 bp amplified cDNA fragment
of p53 is shown in the figure.
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ß -actin amplified fragment
of 289 bp. Lane 1 is
molecular weight markers.
Lanes 2-5 are ß -actin
control PCR product from
guinea pig, human,
mouse and rat pooled
first strand Omni-cDNA™.
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An amplified fragment of 289 bp. Lane 1 is
molecular weight markers. Lanes 2-6 are
ß -actin control PCR products from brain,
liver, intestine, skeletal muscle and spleen.
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First Strand Pooled cDNA
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Product
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Size
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Catalog No.
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Price($)
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Omni-cDNA™ Human
First Strand Pooled cDNA
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5 µg
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10-0100-05
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425.00
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Omni-cDNA™ Mouse
First Strand Pooled cDNA
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5 µg
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10-0200-05
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425.00
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Omni-cDNA™ Rat
First Strand Pooled cDNA
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5 µg
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10-0300-05
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425.00
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Omni-cDNA™ Guinea Pig
First Strand Pooled cDNA
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5 µg
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10-2100-05
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425.00
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Click here to order!
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First Strand cDNA
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Product
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Size
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Catalog No.
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Price($)
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Guinea pig first strand
cDNA; Various tissues;
Brain, Heart,Liver,
Kidney, etc.
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5 µg
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10-21XX-05
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425.00
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Click here to order!
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