Omni-Array™ mRNA Amplification System
A universal method that performs
global mRNA amplification,
while maintaining the
relative proportions comparable
to the original sample is the
Omni-Array™ mRNA amplification
system. This system is
compatible with all labeling and
detection systems. Samples
which were previously thought
to be too small for microarray
or other genome wide study
can now be amplified using the
Omni-Array™ mRNA amplification
system.
APPLICATION:
Microarray labeling, global
mRNA amplification, cDNA
synthesis, RT-PCR, cloning etc.
Omni-Array™ mRNA
Amplification system
The Omni-Array™ mRNA amplification
system provides a rapid and simple
procedure for the generation of
sufficient amounts of high quality
sense or antisense strand RNA using
nanogram quantities of starting RNA.
The Omni-Array™ Amplification
System is especially suited when the
availability of total RNA becomes the
limiting factor in performing certain
experimental procedures.
System Format
The system contains enough
reagents for 5 two round or 10 single
round amplifications. Consistent
results are obtained with 100 ng total
RNA for single round amplification
and with 2 ng total RNA for two
round amplification. The final yield of
mRNA is enough for labeling at least
2 microarrays. A single round of
amplification results in 100-1000-fold
amplification of the entire mRNA
population from the sample.
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One Round Amplification;
2 ng total RNA amplified
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p53 cDNA amplification
from human
Omni-mRNA™ pooled
reference mRNA. Lane
1, mw markers; lanes
2 and 4, ~1.3 kb 5'
end fragment of p53;
lanes 3 and 5, ~500 bp
of middle portion of
p53. Lanes 2-3 and 4-5
represent reproducible
different preps.
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