One tube containing lyophilized oligo linker. The product is supplied as a lyophilized powder. Oligo purity is greater than 98% as determined by denaturing polyacrylamide gel electrophoresis.
Reconstitute oligo linker in sterile water preferably at 1 µg/ µg. Linkers are short oligos that are supplied in an annealed form. Due to the short size the Tm is low and thus even room temperature storage should be avoided. Always keep them on ice when in use.
After reconstitution store at -20oC
Linkers are used for various cloning strategies to introduce restriction sites in the DNA after ligation. Linkers are short synthetic palindromic sequences that self anneal to form blunt ended double stranded fragments. Linkers are supplied as phosphorylated and non-phosphorylated forms.
All Gene Link supplied linkers pass stringent quality control protocols of ligation and cleavage. The product is supplied as a lyophilized powder, after reconstitution store at -20oC. Oligo purity is greater than 98% as determined by denaturing polyacrylamide gel electrophoresis
1. Follow ligase provider protocol for ligation.
2. Visit Gene Link web site for Ligation Calculator to determine ratio of insert:vector at the following link http://www.genelink.com/tools/gl-lc.asp
3. Conditions for linker ligation is the same as for insertion of DNA fragments into a plasmid vector. The recommended molar ratio of phosphorylated linker:dephosphorylated vector is 10-100:1. When using phosphorylated vector, the linker:vector molar ratio should be >100:1.