Do not use for precipitation of RNA smaller than 300 nucleotides.
A final concentration of 2.5M LiCl is effective in precipitation of RNA larger than 300 nucleotides without the addition of ethanol, this method of precipitation selectively precipitates RNA and does not efficiently precipitate DNA, protein or carbohydrate. It is the method of choice for removing inhibitors of translation or cDNA synthesis from RNA preparations. It also provides a simple rapid method for recovering RNA from in vitro transcription reactions. As little as 50 ng of RNA can be quantitatively recovered by centrifugation at 12K rpm for 20 minutes at 4°C.
Usage: 2.5M Lithium Chloride final concentration without addition of ethanol or isopropanol.
Routine precipitation of RNA at Concentrations ≥100 ng/mL.