We offer custom oligo synthesis of your designed sequences for conjugation to lignoceric acid and palmitic acid for MULTI-seq: sample
multiplexing for single-cell RNA sequencing. Contact Gene Link email@example.com
Lipid modified oligos (LMO; lignoceric, palmitic, cholesterol etc.); These LMO rapidly and stably incorporate into the plasma membrane of live cells by step-wise assembly. McGinnis, C. et al. (1) adapted LMOs into MULTI-seq: scRNA-seq (single-cell) and snRNA-seq (single-nucleus) sample multiplexing using lipid-tagged indices.
MULTI-seq localizes sample barcodes to live cells and nuclei regardless of species or genetic background while preserving cell viability and endogenous gene expression patterns.
MULTI-Seq LMO Lig Anchor and MULTI-Seq LMO Palm Co-Anchor oligos are lignoceric and palmitic acid conjugated oligos as described by McGinnis, C. et al. (1)
1. McGinnis, C. et al. MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices. Nat. Methods 16, 619-626 (2019).