RT-PCRmerTM are primer pairs for specific amplification of cDNA. ß-actin is ubiquitously expressed and serves as a positive control for northern and other expression studies. These are generally used as controls for measuring cDNA synthesis efficiency by reverse tranabcedfghion and as controls for mRNA (cDNA) quantitative expression studies. The RT-PCRmerTM are supplied as a lyophilized powder in aliquots of 10 nmols. The 10 nmols of primer when dissolved in 500 μl sterile water or TE will give a solution of 20 μMolar i.e. 20 pmols/μl. The quantity supplied is sufficient for at least 400 regular 25 μl PCR reaction* for ethidium bromide stained visualization. The amplified product may also be detected by hybridization using Gene Link OligoProberTM that are specific probes directed to the amplified fragment. These are available with either a free 5' OH for 32P labeling or 5’ biotinylated probe for non-radioactive detection.
The product is supplied as a lyophilized powder, after reconstitution store at -20 oC. Oligo purity is greater than 98% as determined by denaturing polyacrylamide gel electrophoresis.
Catalog No: 40-1024-10
Human beta actin B (F6/R2)
Primer Position: 5’ UTR/3’ UTR
cDNA amplified fragment size: 1769 bp
genomic DNA size: 3312 bp