Quick Order |All Online Ordering|Product Catalog Ordering|Oligo Modifications List|Product Info & Literature|Oligo Design Tools/Resources

 DMSO (Dimethyl sulfoxide); 1 mL
Catalog No. ProductPDF Guide Manual MSDS SizePrice.Quantity 
40-3031-10DMSO (Dimethyl sulfoxide); 1 mL 1 mL$10.00

PCR additives Betaine, DMSO and formamide reduces the Tm and the complex secondary structure thus the duplex stability. Tetramethyl ammonium chloride (TMAC) actually increases the specificity of hybridization and increases the Tm. The use of TMAC is recommended in PCR conditions using degenerate primers.
These PCR additives and enhancing agents have been used to increase the yield, specificity and consistency of PCR reactions. These additives may have beneficial effects on some amplification and it is impossible to predict which agents will be useful in a particular context and therefore they must be empirically tested for each combination of template and primers.
DMSO (dimethyl sulfoxide) reduce secondary structure and is particularly useful for GC rich templates. DMSO at 2-10% may be necessary for amplification of some templates, however 10% DMSO can reduce Taq polymerase activity by up to 50% so it should not be used routinely.

Related Products
Omni-Marker™ Universal unlabeled; 100 uL
Omni-Marker™ Universal unlabeled; 1 mL
Omni-Marker™ Low unlabeled; 500 uL
Loading buffer 5X BPB/XC non-denaturing; 1 mL
Loading Buffer 5X Orange G/XC non-denaturing; 1 mL
Loading Buffer 2X BPB/XC Denaturing for Sequencing; 1 mL
Omni-Clean™ Gel DNA Beads Purification System; 100 purification
Omni-Clean™ Gel DNA Beads Purification System; 500 purifications
Omni-Clean™ Gel DNA Spin Column Purification System; 100 purifications
Omni-Clean™ Gel DNA Spin Purification; 500 purifications
Omni-Clean™ DNA Concentration System; 100 purifications
Omni-Clean™ DNA Concentration System; 500 purifications
Omni-Clean™ DNA Spin Column Concentration System; 100 purification
Omni-Clean™ DNA Spin Column Concentration System; 500 purification
Taq DNA Polymerase 400 units 5 u/ul; 80 uL; 400 units
Taq PCR Kit; 200 rxns
Taq PCR Kit with Controls; 200 rxns
PCR Master Mix (2X) 100 reactions (2 x 1.3 ml); 100 rxns
PCR Master Mix (2X) 200 reactions (4 x 1.3 ml); 200 rxns
Taq DNA Polymerase 300 units 5 u/ul 60 uL; 300 units
PCR Buffer Standard (10X); 1.6 mL
Taq Polymerase Dilution Buffer; 1 mL
MgCl2 25 mM; 1.6 mL
Primer and Template Mix 500 bp 40 reactions; 100 uL
Nuclease Free Water (DEPC Free) 10 X 1.6 mL; 16 mL
DMSO (Dimethyl sulfoxide); 1 mL
KCl 300 mM; 1 mL
Agarose Tablets 0.5 g X 100; 50 g
50 X TAE buffer; 100 mL
TAE Buffer 50 X Concentrate; 1000 mL
TBE Buffer 5 X Concentrate; 1000 mL
Betaine 5M; 1 mL
Loading Buffer 10X BPB/XC non-denaturing; 1 mL
MgCl2 50 mM; 1.6 mL
dNTP 10mM; 0.5 mL
Nuclease Free Water (DEPC Free); 500 mL
DEPC Treated Water; 10 X 1.6 mL
DEPC Treated Water; 50 mL
DEPC Treated Water; 500 mL
DEPC Treated Water; 1L
Agarose LE Molecular Biology Grade; 100 g
Agarose LE Molecular Biology Grade; 500 g
TMAC (Tetramethyl ammonium chloride) 100 mM; 1 mL
dNTP 2mM (10X); 1.1 mL
Loading buffer 2X BPB/XC Sequencing; 15 mL
Nuclease Free Water (DEPC Free); 50 mL
Nuclease Free Water (DEPC Free); 1 L
Loading buffer 5X BPB/XC non-denaturing; 15 mL
PCR Buffer Mg Free (10 X); 1.6 mL
Agarose LE Molecular Biology Grade 1 Kg; 1000 g
Oligonucleotide Synthesis |  Flourescent Molecular Probes |  Gene Detection Systems |  Tools & Reagents |  Gene Assays |  RNAi
© 2017 Gene Link |  Terms & Conditions |  Licenses |  Privacy Policy |  June 28, 2017 12:09:12 AM