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 Lumisol III,Hybridization Solution for Oligo Probe; 200 mL
Catalog No. ProductPDF Guide
40-5024-20Lumisol III,Hybridization Solution for Oligo Probe; 200 mL

Product Description

Lumisol II & III are ready-to-use hybridization solutions specifically formulated for non-radioactive chemiluminescent hybridization.

Lumisol II Lumisol II is for use with digoxigenin or biotin labeled DNA or RNA probes in Southern & Northern blot protocols. It can also be used with radioactive labeled probes.

Lumisol III Lumisol III is specifically formulated for use with oligonucleotide probes labeled with biotin, digoxigenin, alkaline phosphatase or other detection ligand or enzymes.

Applications Lumisol II hybridization solution can be used for all stringent types of nucleic acid blot hybridization conditions, particularly using non-radioactive digoxigenin labeled probes. Lumisol II hybridization solution can be used for prehybridization and hybridization. The hybridization temperature should be calculated for proper results. Duration of hybridization can be reduced to 6 hours and overnight hybridization can be used for high sensitivity requirements and for convenience. Lumisol III hybridization solution is specifically formulated for oligonucleotide probes that require only 30 minute pre-hybridization and 30 minute hybridization. Longer hybridization is not recommended. The hybridization temperature should be calculated for proper results and should not be more than 55C for alkaline phosphatase labeled oligo probes.

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