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GLanim

Tm Calculation for Oligos

Enter your oligonucleotide sequence: UNDEFINED

Combined concentration of K⁺ and Na⁺ in the reaction: mM

Primer concentration in the reaction: nM

The oligo has a 5' -phosphate group



Sequence Analyzed:   UNDEFINED

% GC:   0

Base length:   9

Molecular Weight:      -61 D

Basic T_m:   0 ° C

Salt adjusted T_m:   NaN ° C

Base-stacking calculated T_m:   NaN ° C
Thermodynamic Parameters:

ΔH    0kcal/mol           ΔS    NaNcal/degree k mol


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