Literature (Product Manuals, Specifications, MSDS)

Catalog No.	Product	PDF Guide
MAG_OLIGO	Magnetic Bead Oligo Conjugation

Custom Magnetic Bead oligo conjugation
Gene Link offers a wide variety of modifications that enable oligonucleotides to be conjugated to various ligands including solid surfaces with the appropriate functional groups. Immuno chemistry based affinity ligands offered by Gene Link are custom oligos modified with Biotin and Digoxigenin. Gene Link offers magnetic beads conjugation for direct hybridization based affinity to complementary sequences. The most common conjugation chemistry involves oligos modified with a primary amino group; the amino group can be placed at 5 or 3 end or any desired internal position using an amino C6 base. The amino group reacts with N-hydroxysuccinimide (NHS) functional groups to form a stable amide linkage; the reaction efficiency varies from 50% to 90% depending on the ligand activated NHS group. The requirement for this conjugation is the availability of the desired ligand or solid surface in an activated NHS form for conjugation to the amino modified oligonucleotide.