Quick Order |All Online Ordering|Product Catalog Ordering|Oligo Modifications List|Product Info & Literature|Oligo Design Tools/Resources

 TMAC (Tetramethyl ammonium chloride) 100 mM; 1 mL
Catalog No. ProductPDF Guide Manual MSDS SizePrice.Quantity 
40-3053-10TMAC (Tetramethyl ammonium chloride) 100 mM; 1 mL 1 mL$13.00

PCR additives Betaine, DMSO and formamide reduces the Tm and the complex secondary structure thus the duplex stability. Tetramethyl ammonium chloride (TMAC) increases the specificity of hybridization and increases the Tm. The use of TMAC is recommended in PCR conditions using degenerate primers.
These PCR additives and enhancing agents have been used to increase the yield, specificity and consistency of PCR reactions. These additives may have beneficial effects on some amplification and it is impossible to predict which agents will be useful in a particular context and therefore they must be empirically tested for each combination of template and primers.
TMAC is used to reduce potential DNARNA mismatch and improve the stringency of hybridization reactions. It increases Tm and minimizes mis-pairing. TMAC is generally used at a final concentration of 15-100mM to eliminate nonspecific priming.

Related Products
Omni-Marker Universal unlabeled; 100 uL
Omni-Marker Universal unlabeled; 1 mL
Omni-Marker Low unlabeled; 500 uL
Loading buffer 5X BPB/XC non-denaturing; 1 mL
Loading Buffer 5X Orange G/XC non-denaturing; 1 mL
Loading Buffer 2X BPB/XC Denaturing for Sequencing; 1 mL
Omni-Clean Gel DNA Beads Purification System; 100 purification
Omni-Clean Gel DNA Beads Purification System; 500 purifications
Omni-Clean Gel DNA Spin Column Purification System; 100 purifications
Oligonucleotide Synthesis |  Flourescent Molecular Probes |  Gene Detection Systems |  Tools & Reagents |  Gene Assays |  RNAi
© 2023 Gene Link |  Terms & Conditions |  Licenses |  Privacy Policy |  November 30, 2023 1:32:11 PM