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Ligation Calculator



PART 1 : Convert double strand DNA (ds DNA) to pmoles and ends
DNA Name
ds DNA size in base pairs (bp)
Quantity in micogram (ug)
PART 2 : Ligation Calculator
Generally ligations are tested at 1:3, 1:1, 3:1 insert:vector molar ratios.
Insert size in base pairs (bp)
Vector size in base pairs (bp)
Vector amount in ng 10   50   100   Other Value:
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