Literature (Product Manuals, Specifications, MSDS)

Lumisol III,Hybridization Solution for Oligo Probe; 125 mL

Catalog No.	Product	PDF Guide
40-5024-80	Lumisol III,Hybridization Solution for Oligo Probe; 125 mL

Product Description

Lumisol� II & III are ready-to-use hybridization solutions specifically formulated for non-radioactive chemiluminescent hybridization.

Lumisol� II Lumisol� II is for use with digoxigenin or biotin labeled DNA or RNA probes in Southern & Northern blot protocols. It can also be used with radioactive labeled probes.

Lumisol� III Lumisol� III is specifically formulated for use with oligonucleotide probes labeled with biotin, digoxigenin, alkaline phosphatase or other detection ligand or enzymes.

Applications Lumisol� II hybridization solution can be used for all stringent types of nucleic acid blot hybridization conditions, particularly using non-radioactive digoxigenin labeled probes. Lumisol� II hybridization solution can be used for prehybridization and hybridization. The hybridization temperature should be calculated for proper results. Duration of hybridization can be reduced to 6 hours and overnight hybridization can be used for high sensitivity requirements and for convenience. Lumisol� III hybridization solution is specifically formulated for oligonucleotide probes that require only 30 minute pre-hybridization and 30 minute hybridization. Longer hybridization is not recommended. The hybridization temperature should be calculated for proper results and should not be more than 55�C for alkaline phosphatase labeled oligo probes.