SmartBase™ siRNA Modifications: Design Guide for Enhanced siRNA Performance.
Gene Link presents various design options for synthesizing effective siRNAs, probes and oligos based on the
application. SmartBase™ siRNA can be synthesized in a predetermined way to exhibit the features that is desired;
for instance to increase duplex stability 2’-fluoro C and U bases and 5-methyl dC and 2-Amino dA can be
substituted, for nuclease resistance the phosphodiester linkages can be selectively substituted with
phosphorothioate and for cellular delivery we may add cholesterol to the synthetic oligonucleotide sequence or
modify with thiol or amine for post synthesis conjugation with Cell Penetrating Peptides (CPP’s) that are known
to aid transport and facilitate cellular uptake.
The premise of this product guide is to introduce the use of SmartBase™ modifications and go beyond the
traditional use of standard DNA and RNA bases for constructing synthetic siRNAs in particular and as well
emphasize their use in primers, oligos and probes. SmartBase™ modifications also introduces the molecular
biologists to develop a cross-disciplinary synergy of molecular applications to a wealth of nucleic acid chemistry
tools available as modified bases to impart specific properties compatible with biological applications and gene