Lumisol™ II & III are ready-to-use hybridization solutions specifically formulated for non-radioactive chemiluminescent hybridization.
Lumisol™ II is for use with digoxigenin or biotin labeled DNA or RNA probes in Southern & Northern blot protocols. It can also be used with radioactive labeled probes.
Lumisol™ III is specifically formulated for use with oligonucleotide probes labeled with biotin, digoxigenin, alkaline phosphatase or other detection ligand or enzymes.
Lumisol™ II hybridization solution can be used for all stringent types of nucleic acid blot hybridization conditions, particularly using non-radioactive digoxigenin labeled probes. Lumisol™ II hybridization solution can be used for prehybridization and hybridization. The hybridization temperature should be calculated for proper results. Duration of hybridization can be reduced to 6 hours and overnight hybridization can be used for high sensitivity requirements and for convenience.
Lumisol™ III hybridization solution is specifically formulated for oligonucleotide probes that require only 30 minute pre-hybridization and 30 minute hybridization. Longer hybridization is not recommended. The hybridization temperature should be calculated for proper results and should not be more than 55°C for alkaline phosphatase labeled oligo probes.