Gel Loading Buffer 5X BPB/XC and 10X BPB/XC are concentrates that contain bromophenol blue and xylene cyanol as tracking dyes. The migration of the tracking dyes differs in different percentage gels. See table below for approximate co-migration of dyes with DNA base pair sizes. In agarose gels over a range of 0.7% to 1.4% (w/v), xylene cyanol co-migrates with ~4 kb linear double strand DNA and bromophenol blue co-migrates with ~300 bp double strand DNA. In a 12% polyacrylamide gel xylene cyanol co-migrates with approximately 70 bp DNA and bromophenol blue co-migrates with ~20 bp DNA.
Suitable for use in nucleic acid gel electrophoresis.
DNase, RNase: None detected.